Ue T, Yokozaki H, Kagai K, Higashikawa K, Yasui W, Sugiyama M, Tahara E, Ishikawa T
Department of Oral and Maxillofacial Surgery II, Hiroshima University School of Dentistry, Japan.
J Oral Pathol Med. 1998 May;27(5):197-201. doi: 10.1111/j.1600-0714.1998.tb01941.x.
In the present study, we examined the expression of CD44 variant exons in human oral squamous cell carcinoma (OSCC). Of ten cell lines from OSCCs, two (KB and H357), as well as the HeLa cell line, failed to express CD44 variant exons. In surgical specimens, all normal mucosa expressed CD44v9 (both mRNA and protein). Of 40 primary OSCCs, 19 (47.5%) showed downregulation of CD44v9, which correlated with tumor cell differentiation, primary metastasis to lymph nodes and secondary metastasis to lymph nodes. The results suggest that the downregulation of CD44v9 may play a role in lymphatic metastasis of OSCC and changes in its expression may be a useful diagnostic tool to determine the metastatic potential of OSCC to lymph nodes. Moreover, three cell lines that failed to express CD44 variant exons might become a useful experimental model to study the role of variant exons in the progression of OSCC.
在本研究中，我们检测了人口腔鳞状细胞癌（OSCC）中CD44可变外显子的表达。在10种OSCC细胞系中，有两种（KB和H357）以及HeLa细胞系未能表达CD44可变外显子。在手术标本中，所有正常黏膜均表达CD44v9（mRNA和蛋白）。在40例原发性OSCC中，19例（47.5%）显示CD44v9下调，这与肿瘤细胞分化、淋巴结原发性转移和继发性转移相关。结果表明，CD44v9下调可能在OSCC的淋巴转移中起作用，其表达变化可能是确定OSCC向淋巴结转移潜能的有用诊断工具。此外,三种未能表达CD44可变外显子的细胞系可能成为研究可变外显子在OSCC进展中作用的有用实验模型。
