Harada H, Mitsuyasu T, Nakamura N, Higuchi Y, Toyoshima K, Taniguchi A, Yasumoto S
Second Department of Oral Anatomy and Cell Biology, Kyushu Dental College, Kitakyushu, Japan.
J Oral Pathol Med. 1998 May;27(5):207-12. doi: 10.1111/j.1600-0714.1998.tb01943.x.
Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.
成釉细胞瘤是生长缓慢、具有局部侵袭性且有潜在破坏性行为的肿瘤。调节成釉细胞瘤细胞生长和侵袭的分子机制尚不清楚。由于培养的成釉细胞瘤细胞寿命非常有限，建立成釉细胞瘤细胞的永生化克隆将有助于其研究。我们使用16型人乳头瘤病毒制备了一种永生化的成釉细胞瘤细胞系（AM-1）。该细胞系保持上皮细胞形态，并表达细胞角蛋白K8、K14、K18、K19。此外，还表达了防止细胞凋亡的bcl-2蛋白。我们在体外研究了这些细胞在胶原基质上的行为。这些细胞在胶原降解灶上呈单层生长，并可在这些部位侵入胶原凝胶。由于AM-1细胞系的行为模拟了成釉细胞瘤在体内的行为，它可能是研究这些肿瘤的一个有价值的模型。
