Tao Qian, Huang Hongzhang
Department of Oral and Maxillofacial Surgery, Guanghua Stomatological Faculty, Sun Yat-sen University, Guangzhou 510060, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2002 May;37(3):167-9.
To establish an immortalized ameloblastoma cell line.
The primary cultured ameloblastoma cells were transfected with pRSV-Tag using Transfect AMINE kit. Tansfected cells were passaged to pass through crisis period and immortalize.
Cultured ameloblastoma cells were composed predominantly of closely packed small polygonal cells with epithelial morphology. They had limited life-span of 51 days in vitro. The small polygonal cells were eventually replaced by large flattened cells and subsequently became senescent and dead. On the other side, those tumor cells transfected with SV40Tag could live for a longer time. The majority of them died in crisis period while the survived cells from crisis period gained the ability to proliferate. There was no morphological change in TAM-1 compared with original cultured cells. A cell clone was harvested which was alive and keeping on proliferating after having been subcultured for 25 times. It was named TAM-1. The epithelial origin of TAM-1 was confirmed by strong immunoreactivity for cytokeratin in contrast to negative vimentin expression. It was detected that SV40Tag had been transfected into TAM-1 genesome and expressed continuously by PCR and RT-PCR.
TAM-1 is immortalized ameloblastoma cell line in vitro.
建立永生化成釉细胞瘤细胞系。
采用Transfect AMINE试剂盒，用pRSV-Tag转染原代培养的成釉细胞瘤细胞。将转染后的细胞传代以度过危机期并实现永生化。
培养的成釉细胞瘤细胞主要由紧密排列的小多边形细胞组成，具有上皮形态。它们在体外的寿命有限，为51天。小多边形细胞最终被大的扁平细胞取代，随后衰老死亡。另一方面，那些用SV40Tag转染的肿瘤细胞可以存活更长时间。它们中的大多数在危机期死亡，而从危机期存活下来的细胞获得了增殖能力。与原代培养细胞相比，TAM-1没有形态学变化。收获了一个细胞克隆，该克隆在传代培养25次后仍存活并持续增殖。它被命名为TAM-1。通过细胞角蛋白的强免疫反应性与波形蛋白表达阴性对比，证实了TAM-1的上皮来源。通过PCR和RT-PCR检测到SV40Tag已转染到TAM-1基因组中并持续表达。
TAM-1是体外永生化的成釉细胞瘤细胞系。
