来自嗜热蓝藻聚球藻（Synechococcus vulcanus）的一种编码与α-晶状体蛋白相关的小热休克蛋白的基因的克隆、表征及转录分析。
Cloning, characterization, and transcriptional analysis of a gene encoding an alpha-crystallin-related, small heat shock protein from the thermophilic cyanobacterium Synechococcus vulcanus.
作者信息
Roy S K, Nakamoto H
机构信息
Department of Biochemistry and Molecular Biology, Saitama University, Urawa 338, Japan.
出版信息
J Bacteriol. 1998 Aug;180(15):3997-4001. doi: 10.1128/JB.180.15.3997-4001.1998.
Abstract
hspA, a gene encoding a 16-kDa heat-induced protein from the thermophilic cyanobacterium Synechococcus vulcanus, has been cloned and sequenced. The deduced amino acid sequence of the gene product showed significant homology to sequences of the family of alpha-crystallin-related, small heat shock proteins. A monocistronic mRNA of hspA increased transiently in response to heat shock. The heat shock induction occurred at a vegetative promoter but without the CIRCE (controlling inverted repeat of chaperone expression) element.
摘要
hspA是一个来自嗜热蓝藻温泉聚球藻（Synechococcus vulcanus）的编码16 kDa热诱导蛋白的基因，已被克隆和测序。该基因产物推导的氨基酸序列与α-晶状体蛋白相关的小热休克蛋白家族的序列显示出显著的同源性。hspA的单顺反子mRNA在热休克反应中短暂增加。热休克诱导发生在营养启动子处，但没有伴侣蛋白表达的控制反向重复序列（CIRCE）元件。
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