Yu H, Ahmed H, Vasta G R
Veridian Corporation, 5200 Springfield Pike, Suite 200, Dayton, OH 45431-1289, USA.
Anal Biochem. 1998 Jul 15;261(1):1-7. doi: 10.1006/abio.1998.2614.
A magnetic microplate chemifluorimmunoassay (MMCIA) is described using an immunomagnetic separation and a fluorescent microplate technique for rapid detection of low-level Escherichia coli O157:H7, Bacillus subtilis var. niger spores, and Staphylococcal enterotoxin type B from whole blood. In general, the MMCIA has at least several-fold more sensitivity than the conventional enzyme-linked immunosorbent assay. In addition, the assay sensitivities using direct fluorochrome label as the reporter, or alkaline phosphatase (AP) with various assay substrates, such as pNPP and AttoPhos, were assessed.
