Llewellyn L E, Doyle J, Negri A P
Australian Institute of Marine Science, Townsville 4810, Australia.
Anal Biochem. 1998 Jul 15;261(1):51-6. doi: 10.1006/abio.1998.2707.
An isoform of the paralytic shellfish poison (PSP)-specific receptor saxiphilin, from the tropical centipede Ethmostigmus rubripes, was used as the basis for a radiometric, high-throughput, microtiter plate assay for this group of toxins. Characterization of the assay revealed that it was able to detect several representatives from the various structural PSP subgroups and yet was insensitive toward tetrodotoxin. To test the utility of the assay as a seafood toxin-monitoring tool, the assay was subjected to a variety of marine organism extracts, some of which were known to contain PSPs, and whole extract toxicity expressed as STX equivalents (STXeq) was measured by two methods: First, by comparison of values from a screening assay with a standard STX inhibition curve and, second, for highly active extracts, by calculation using the IC50 from a full inhibition curve of the extract. For extracts which could be quantified by both methods, there was almost 100% correlation between the derived values. STXeq derived by both methods from the bioassay highly correlated with absolute toxin quantities from HPLC analysis.
来自热带蜈蚣红巨龙蜈的麻痹性贝类毒素（PSP）特异性受体saxiphilin的一种同工型，被用作针对这类毒素的放射性、高通量、微孔板检测方法的基础。对该检测方法的特性分析表明，它能够检测出各种结构的PSP亚组中的几种代表物，并且对河豚毒素不敏感。为了测试该检测方法作为海鲜毒素监测工具的实用性，将该检测方法应用于多种海洋生物提取物，其中一些已知含有PSP，并通过两种方法测量以石房蛤毒素当量（STXeq）表示的全提取物毒性：第一，通过将筛选检测的值与标准石房蛤毒素抑制曲线进行比较；第二，对于高活性提取物，通过使用提取物完全抑制曲线的IC50进行计算。对于可以通过两种方法进行定量的提取物，所得值之间几乎存在100%的相关性。通过生物检测由两种方法得出的STXeq与HPLC分析得出的绝对毒素量高度相关。
