Courgnaud V, Saurin W, Villinger F, Sonigo P
Génétique des Virus, ICGM-CNRS UPR415, Institut Cochin de Génétique Moléculaire, Paris, France.
Virology. 1998 Jul 20;247(1):41-50. doi: 10.1006/viro.1998.9217.
To address the mechanisms of host-virus adaptation and pathogenesis of lentiviral infections, we compared the evolution of the same isolate of simian immunodeficiency virus (SIVsmm9) in two different situations: nonpathogenic infection of its natural host, the sooty mangabey, and AIDS-inducing infection of a new host, the rhesus macaque. Samples were obtained at 6, 12, and 23 or 30 months postinfection from three animals of each species. Sequences were derived from the V1 and V2 domains of the surface glycoprotein. In the macaques, we observed specific variations absent from all mangabey samples, indicating that different host species select different virus variants. In the macaques, we also observed a different shape in the phylogenetic tree, a lower divergence of sibling sequences, and a lower synonymous/nonsynonymous change ratio than in the mangabeys. This suggests that the viral population is larger and submitted to weaker selection pressures when host-virus adaptation is achieved, such as in the mangabey.
为了探究慢病毒感染中宿主-病毒适应性及发病机制，我们比较了同一株猴免疫缺陷病毒（SIVsmm9）在两种不同情况下的进化情况：在其天然宿主乌黑白眉猴中的非致病性感染，以及在新宿主恒河猴中的致艾滋病感染。在感染后6个月、12个月以及23或30个月时，从每个物种的三只动物身上采集样本。序列来自表面糖蛋白的V1和V2结构域。在恒河猴中，我们观察到所有白眉猴样本中均不存在的特定变异，这表明不同的宿主物种选择不同的病毒变体。在恒河猴中，我们还观察到系统发育树的形状不同，姐妹序列的分歧较低，并且与白眉猴相比，同义/非同义变化率较低。这表明当宿主-病毒适应性达成时，例如在白眉猴中，病毒群体更大且受到的选择压力较弱。
