Bowring F J, Catcheside D E
School of Biological Sciences, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia.
Curr Genet. 1998 Jul;34(1):43-9. doi: 10.1007/s002940050364.
In cross B163, heteroallelic am1 am6 and heterozygous for both conventional genetic flanking markers and closer molecular markers, we previously found that the majority on flanker exchanges were remote from events that generated prototrophic recombinants. We report here that natural polymorphisms distinguishing the parents of cross B163 also include sequences within and closely flanking am. Segregation of these markers in B163 prototrophs confirms that the majority of meiotic recombination events at am resulted from gene conversion. Conversion of am6, the distal allele, is more frequent than conversion of am1. Twelve percent of tracts in am6 convertants were discontinuous while 30% of continuous tracts converting am6 extend less than 741 bp.
在杂交B163中，它是异等位基因am1 am6，并且对传统遗传侧翼标记和更近的分子标记均为杂合，我们之前发现侧翼交换中的大多数都远离产生原养型重组体的事件。我们在此报告，区分杂交B163亲本的天然多态性还包括am内部和紧密侧翼的序列。这些标记在B163原养型中的分离证实，am处的大多数减数分裂重组事件是由基因转换导致的。远端等位基因am6的转换比am1的转换更频繁。am6转化体中12%的片段是不连续的，而转换am6的连续片段中有30%延伸不到741 bp。
