Kosman D, Small S, Reinitz J
Brookdale Center for Molecular Biology, Box 1126, Mt. Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
Dev Genes Evol. 1998 Jul;208(5):290-4. doi: 10.1007/s004270050184.
We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1(2)/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.
我们描述了一种从细菌表达的蛋白质中快速制备一组高质量多克隆抗体的方法。每种蛋白质大约需要1（2）/3天的制备时间。加速该过程的一个步骤是通过沉淀十二烷基硫酸钠（SDS）来可视化纯化的条带。在双蒸水中沉淀可提高纯化重组蛋白的抗原性。展示了使用所得血清对果蝇胚胎进行荧光染色的结果。
