Patterson A H, McManus D T, Maxwell P
Immunocytochemistry and Molecular Pathology Laboratory, Royal Victoria. Hospital, Northern Ireland, UK.
Br J Biomed Sci. 1998 Mar;55(1):2-7.
This study sought to refine a fluorescence in situ hybridisation (FISH) technique for the study of numerical chromosome aberrations in cells recovered from breast tumours by fine-needle aspiration (FNA). Such techniques have been used to study numerical aberrations of chromosomes from tumours from many sites. This technique is intended for use in a routine cytopathology laboratory, and no specialised cytogenetics knowledge is necessary. Slide preparation, slide and probe denaturation, and stringency conditions were investigated. We found that the simplest slide preparation technique (direct smears) gave the best results. No difference was seen when slide and probe were denatured together or separately. Stringency conditions must be adapted to suit particular probes. Study of numerical chromosomal changes may help determine their significance in the clinical progression of the disease. Such changes may prove to have prognostic significance and therefore influence treatment of breast cancer. FISH on interphase cells is a technically straightforward, reproducible technique, suited to the study of numerical chromosome aberrations in tumour cells. The method has the potential for use in the study of malignant cells from sites other than breast, including ovary, prostate, lung and bladder.
本研究旨在优化一种荧光原位杂交（FISH）技术，用于研究通过细针穿刺（FNA）从乳腺肿瘤中获取的细胞的染色体数目畸变。此类技术已被用于研究来自许多部位肿瘤的染色体数目畸变。该技术旨在用于常规细胞病理学实验室，无需专业的细胞遗传学知识。研究了玻片制备、玻片和探针变性以及严谨性条件。我们发现最简单的玻片制备技术（直接涂片）效果最佳。玻片和探针一起变性或分别变性未见差异。严谨性条件必须根据特定探针进行调整。研究染色体数目变化可能有助于确定其在疾病临床进展中的意义。此类变化可能具有预后意义，从而影响乳腺癌的治疗。间期细胞的FISH是一种技术上简单、可重复的技术，适用于研究肿瘤细胞中的染色体数目畸变。该方法有可能用于研究除乳腺外其他部位的恶性细胞，包括卵巢、前列腺、肺和膀胱。
