Ichikawa D, Hashimoto N, Hoshima M, Yamaguchi T, Sawai K, Nakamura Y, Takahashi T, Abe T, Inazawa J
Department of Hygiene, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Cancer. 1996 May 15;77(10):2064-9. doi: 10.1002/(SICI)1097-0142(19960515)77:10<2064::AID-CNCR15>3.0.CO;2-T.
Biopsy by fine-needle, aspiration has become a routine technique for the diagnosis of a dominant breast mass. In this study, fluorescent in situ, hybridization (FISH) analysis of interphase nuclei allowed the authors to detect genetic aberrations that are difficult to identify by conventional cytology.
To investigate ways of minimizing misdiagnosis of the cytology of breast tumors, and detecting genetic aberrations preoperatively, the authors performed FISH with specimens obtained by fine-needle aspiration biopsies of 106 primary breast tumors (78 primary breast cancers, 2 phyllodes tumors, and 26 benign breast tumors). Numerical chromosome aberrations were investigated using 3-color FISH performed with (peri)centromere-specific probes for chromosomes 1, 11, and 17.
Sufficient materials for FISH analysis were obtained with aspiration biopsy from 98 of the 106 breast tumors (93.4%). None of the benign tumors nor phyllodes tumors showed evidence of aneusomy for any of the 3 chromosomes. However, 71 of the 74 breast cancers (95.9%) for which sufficient material was available demonstrated aneusomy of at least 1 of the 3 chromosomes tested. The FISH analysis also suggested a possible correlation between aneusomy of chromosome 17 and metastasis to regional lymph nodes (chi-square test = 7.78; P < 0.05).
FISH analysis of fine-needle aspiration biopsies can be a practical and useful method for the preoperative diagnosis of breast carcinoma.
细针穿刺活检已成为诊断乳腺优势肿块的常规技术。在本研究中，对间期核进行荧光原位杂交（FISH）分析使作者能够检测到常规细胞学难以识别的基因畸变。
为了研究将乳腺肿瘤细胞学误诊降至最低以及术前检测基因畸变的方法，作者对106例原发性乳腺肿瘤（78例原发性乳腺癌、2例叶状肿瘤和26例乳腺良性肿瘤）细针穿刺活检获取的标本进行了FISH检测。使用针对1号、11号和17号染色体的（着丝粒）特异性探针进行三色FISH检测，研究染色体数目畸变情况。
106例乳腺肿瘤中有98例（93.4%）通过穿刺活检获得了足够用于FISH分析的材料。良性肿瘤和叶状肿瘤均未显示这3条染色体中任何一条存在非整倍体证据。然而，74例乳腺癌中有71例（95.9%）获得了足够材料，显示至少1条检测的3条染色体存在非整倍体。FISH分析还提示17号染色体非整倍体与区域淋巴结转移之间可能存在相关性（卡方检验=7.78；P<0.05）。
细针穿刺活检的FISH分析可成为乳腺癌术前诊断实用且有用的方法。
