Ioakim-Liossi A, Markopoulos C, Karakitsos P, Safioleas M, Gogas J, Vaiopoulos G
Department of Pathology, Medical School, University of Athens, Greece.
Acta Cytol. 1998 Jul-Aug;42(4):918-22. doi: 10.1159/000331968.
To investigate p53 protein expression in imprints from benign and ductal breast carcinoma cases in relation to the histologic grade of malignancy and clinical stage.
The study group consisted of 60 cases of primary ductal breast carcinomas and 20 benign lesions. For the demonstration of p53 protein expression, an immunocytochemical avidin-extravidin complex technique was applied. Monoclonal antibody p53 was used as the primary antibody, diaminobenzidine as the chromogen and hematoxylin as the counterstain.
Forty-five percent of breast cancer cases showed positive expression of p53. A statistically significant difference in p53 protein expression was observed between grade 1, 2 and 3 carcinomas and stage I, II and III cases. All benign lesions were negative for p53 protein expression.
Immunocytochemical p53 protein expression in cytologic material is a simple method that can be applied in routine cytologic laboratories for the identification of genetic alterations in primary ductal breast cancer.
研究乳腺良性病变及导管癌印片标本中p53蛋白表达与肿瘤组织学分级及临床分期的关系。
研究组包括60例原发性乳腺导管癌及20例良性病变。采用免疫细胞化学抗生物素蛋白-抗生物素蛋白复合物技术检测p53蛋白表达。以单克隆抗体p53为一抗，二氨基联苯胺为显色剂，苏木精为复染剂。
45%的乳腺癌病例显示p53阳性表达。Ⅰ、Ⅱ、Ⅲ级癌及Ⅰ、Ⅱ、Ⅲ期病例的p53蛋白表达存在统计学显著差异。所有良性病变的p53蛋白表达均为阴性。
免疫细胞化学检测细胞材料中p53蛋白表达是一种简单的方法，可应用于常规细胞学实验室，以识别原发性乳腺导管癌的基因改变。
