Callan M B, Shofer F S, Wojenski C, Giger U
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6010, USA.
Thromb Haemost. 1998 Jul;80(1):176-80.
The effects of Chrono-lume (CL) and magnesium sulfate (Mg2+), a component of this luciferin-luciferase reagent, on platelet aggregation were studied in platelet-rich plasma (PRP) obtained from blood anticoagulated with sodium citrate from humans, dogs, cats, horses, and cows. The final added Mg2+ concentration of both solutions ranged from 0.75-3.7 mM. CL and Mg2+ had no effect on maximum aggregation of platelets from humans induced by sub-threshold concentrations of collagen and ADP. In contrast, addition of CL or Mg2+ to canine PRP resulted in a dose-dependent and equal potentiation of platelet aggregation in response to sub-threshold concentrations of collagen, ADP, and thrombin in normal and thrombopathic dogs. The effect of CL on platelet aggregation induced by sub-threshold concentrations of agonists was less pronounced and varied in other species according to the agonist. The reason for the marked difference in sensitivity of human and canine platelets to CL or Mg2+ is not clear, although a difference in releasable cation pools of the platelets from these two species has been recognized. Platelet aggregation studies of animals with suspected thrombopathias should be performed without CL to prevent masking of a platelet function defect.
在从用柠檬酸钠抗凝的人、狗、猫、马和牛的血液中获取的富血小板血浆（PRP）中，研究了Chrono-lume（CL）和这种荧光素-荧光素酶试剂的一种成分硫酸镁（Mg2+）对血小板聚集的影响。两种溶液最终添加的Mg2+浓度范围为0.75 - 3.7 mM。CL和Mg2+对亚阈值浓度的胶原蛋白和ADP诱导的人血小板最大聚集没有影响。相比之下，在正常和血栓形成异常的狗中，向犬PRP中添加CL或Mg2+会导致对亚阈值浓度的胶原蛋白、ADP和凝血酶的血小板聚集呈剂量依赖性且同等程度的增强。CL对亚阈值浓度激动剂诱导的血小板聚集的影响在其他物种中不太明显，并且根据激动剂的不同而有所变化。尽管已经认识到这两个物种的血小板可释放阳离子池存在差异，但尚不清楚人和犬血小板对CL或Mg2+敏感性存在显著差异的原因。对疑似血栓形成异常的动物进行血小板聚集研究时，应在不使用CL的情况下进行，以防止掩盖血小板功能缺陷。
