Vasiljeva I, Kozlovska T, Cielens I, Strelnikova A, Kazaks A, Ose V, Pumpens P
Biomedical Research and Study Centre, University of Latvia, Riga.
FEBS Lett. 1998 Jul 10;431(1):7-11. doi: 10.1016/s0014-5793(98)00716-9.
The new protein carrier was developed on the basis of recombinant RNA phage Qbeta capsid. C-terminal UGA extension of the short form of Qbeta coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qbeta particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31-DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qbeta particles and ensured specific antigenicity and immunogenicity.
新型蛋白质载体是在重组RNA噬菌体Qβ衣壳的基础上开发的。Qβ衣壳短形式的C末端UGA延伸，即所谓的A1延伸，作为在嵌合Qβ颗粒外表面呈递外源肽的靶点。在增强UGA抑制的条件下，嵌合颗粒中A1延伸型与短衣壳的比例从48%降至14%，同时A1延伸的长度增加。一个模型插入片段，即乙型肝炎表面抗原的短前S1表位31-DPAFR-35，在嵌合Qβ颗粒上显示出表面定位，并确保了特异性抗原性和免疫原性。
