Kozlovska T M, Cielens I, Vasiljeva I, Strelnikova A, Kazaks A, Dislers A, Dreilina D, Ose V, Gusars I, Pumpens P
Biomedical Research and Study Centre, University of Latvia, Riga, Latvia.
Intervirology. 1996;39(1-2):9-15. doi: 10.1159/000150469.
The Q beta gene C has been proposed as a new carrier for the exposure of foreign peptide sequences. Contrary to well-known 'display vectors' on the basis of coat proteins of RNA phage group I, group III phage Q beta-based vectors suggested application of the 195-amino acid extension of coat protein (CP) within the so-called A1 protein for insertion of the appropriate immunological epitopes. 'Mosaic' capsids presenting model hepatitis B virus preS1 and HIV-1 gp120 epitopes and formed by Q beta CP together with A1-derived proteins were obtained as a result of (1) suppression of leaky UGA stop codon of the CP gene and (2) simultaneous expression of 'pure' CP and full-length A1-derived genes obtained after the changing of CP-terminating UGA to strong UAA stop codon or sense GGA codon, respectively.
有人提出将Qβ基因C作为一种用于展示外源肽序列的新载体。与基于第一组RNA噬菌体外壳蛋白的知名“展示载体”不同，基于第三组噬菌体Qβ的载体建议在所谓的A1蛋白中利用外壳蛋白（CP）的195个氨基酸延伸部分来插入合适的免疫表位。通过以下方式获得了呈现乙型肝炎病毒前S1和HIV-1 gp120表位模型且由Qβ CP与A1衍生蛋白形成的“镶嵌”衣壳：（1）抑制CP基因的泄漏UGA终止密码子；（2）分别将CP终止的UGA改变为强UAA终止密码子或有义GGA密码子后，同时表达“纯”CP和全长A1衍生基因。
