Nakayama K
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
Leuk Lymphoma. 1998 Apr;29(3-4):399-405. doi: 10.3109/10428199809068576.
IL-6, its receptor and its transducer protein gp130 mRNAs were analyzed in three cell lines with megakaryocytic properties including CMK, K562, and HEL. Their colony proliferation was also assayed in the methylcellulose medium with or without IL-6. CMK had IL-6, IL-6 receptor and gp130 mRNAs, while the other two cell lines lacked IL-6 receptor mRNA expression. K562 responded to IL-6 indicating that it has an alternative receptor to the so called "IL-6 receptor". HEL was not sensitive to IL-6 suggesting that it proliferates with growth factors other than IL-6 via gp130.
在三种具有巨核细胞特性的细胞系（包括CMK、K562和HEL）中分析了白细胞介素-6（IL-6）、其受体及其转导蛋白gp130的信使核糖核酸（mRNA）。还在含有或不含有IL-6的甲基纤维素培养基中检测了它们的集落增殖情况。CMK具有IL-6、IL-6受体和gp130的mRNA，而其他两种细胞系缺乏IL-6受体mRNA表达。K562对IL-6有反应，表明它有一个与所谓的“IL-6受体”不同的受体。HEL对IL-6不敏感，提示它通过gp130依赖于IL-6以外的生长因子进行增殖。
