隐形眼镜消毒系统的杀阿米巴原虫功效的初步研究。
Preliminary studies on the amoebicidal efficacy of contact lens disinfection systems.
作者信息
Stapleton F, Harmis N, Deshpande R, Tran D
机构信息
School of Optometry, University of New South Wales, Sydney, Australia.
出版信息
Aust N Z J Ophthalmol. 1998 May;26 Suppl 1:S44-6. doi: 10.1111/j.1442-9071.1998.tb01369.x.
PURPOSE
This preliminary study evaluated the efficacy of contact lens disinfection systems against Acanthamoeba castellanii, tested with and without co-incubation with Pseudomonas aeruginosa.
METHODS
First, disinfection systems containing 3% hydrogen peroxide, 0.00005% polyaminopropyl biguanide or 0.001% polyquaternium were challenged with A. castellanii for 72 h. Surviving cells were enumerated. Second, A. castellanii were co-incubated with P. aeruginosa for 48 h, then exposed to the disinfectants for 6 h.
RESULTS
There was zero survival of A. castellanii in hydrogen peroxide and variable survival in multipurpose systems. Following co-incubation, A. castellanii survival increased significantly (P = 0.015).
CONCLUSIONS
Multipurpose systems have variable anti-amoeba activity and storage cases contaminated by bacteria may enhance amoeba survival.
目的
本初步研究评估了隐形眼镜消毒系统对卡氏棘阿米巴的有效性，分别在有和没有与铜绿假单胞菌共同孵育的情况下进行测试。
方法
首先，将含有3%过氧化氢、0.00005%聚氨基丙基双胍或0.001%聚季铵盐的消毒系统用卡氏棘阿米巴攻击72小时。对存活细胞进行计数。其次，将卡氏棘阿米巴与铜绿假单胞菌共同孵育48小时，然后暴露于消毒剂中6小时。
结果
过氧化氢中卡氏棘阿米巴的存活率为零，多功能系统中的存活率各不相同。共同孵育后，卡氏棘阿米巴的存活率显著增加（P = 0.015）。
结论
多功能系统具有可变的抗阿米巴活性，被细菌污染的储存盒可能会提高阿米巴的存活率。
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