Arthanari H, Basu S, Kawano T L, Bolton P H
Chemistry Department, Wesleyan University, Middletown, CT 06459, USA.
Nucleic Acids Res. 1998 Aug 15;26(16):3724-8. doi: 10.1093/nar/26.16.3724.
Fluorescent dyes which are specific for duplex DNA have found a wide range of applications from staining gels to visualization of chromosomes. Porphyrin dyes have been found which are highly fluorescent in the presence of quadruplex but not duplex DNA. These dyes may offer a route to the specific detection of quadruplex DNA under biologically important conditions. There are three types of DNA quadruplex structures, and these may play important roles in telomere, centromere, triplet repeat, integration sites and other DNAs, and this first set of porphyrin dyes show some selectivity between the quadruplex types.
对双链DNA具有特异性的荧光染料已在从凝胶染色到染色体可视化等广泛领域得到应用。现已发现卟啉染料，其在存在四链体而非双链DNA时具有高荧光性。这些染料可能为在生物学重要条件下特异性检测四链体DNA提供一条途径。DNA四链体结构有三种类型，它们可能在端粒、着丝粒、三联体重复序列、整合位点及其他DNA中发挥重要作用，并且这第一组卟啉染料在四链体类型之间显示出一定的选择性。
