Rena G, Houslay M D
Division of Biochemistry and Molecular Biology, Davidson Building, IBLS, University of Glasgow, Glasgow G12 8QQ, UK.
Nucleic Acids Res. 1998 Aug 15;26(16):3867-8. doi: 10.1093/nar/26.16.3867.
A method has been devised whereby certain complete restriction digestions can have short overlaps of unique sequence incorporated into the fragments during cloning. Thus one can identify when the DNA fragments are contiguous. Here, this technique is used to produce a contig for a 2.5 kb fragment of genomic DNA. This is a simple approach to ordering digestion fragments without necessarily performing restriction mapping. It is envisaged that this technique will be useful for sequencing cDNAs and small genomic fragments.
已经设计出一种方法，通过该方法某些完全酶切消化可以在克隆过程中将独特序列的短重叠部分整合到片段中。这样就可以识别DNA片段何时是连续的。在此，该技术用于构建2.5 kb基因组DNA片段的重叠群。这是一种简单的方法来排列酶切片段，而不必进行限制性图谱分析。预计该技术将对cDNA和小基因组片段的测序有用。
