Asami K, Gheorghiu E, Yonezawa T
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611, Japan.
Biochim Biophys Acta. 1998 Jul 23;1381(2):234-40. doi: 10.1016/s0304-4165(98)00033-6.
Dielectric behavior of budding yeast in cell separation was studied by comparing two types of temperature-sensitive cell division cycle mutants that arrest before and after cell separation at the restrictive temperature. Single spherical cells before budding but after cell separation showed one main dielectric dispersion (centered at about 1 MHz) and one additional dielectric dispersion (at about 20 MHz), which were the Maxwell-Wagner dispersions due to the plasma membrane and due to both of the cell wall and vacuole, respectively. With cells that accumulated as doublets at a point immediately before cell separation, one more dielectric dispersion appeared around 200 kHz in addition to two dielectric dispersions similar to those found for the single cells without bud. The difference in dielectric behavior between the two types of cells might be mainly attributed to the difference in cell shape, which was theoretically examined using non-spherical cell models.
通过比较两种温度敏感型细胞分裂周期突变体，研究了出芽酵母在细胞分离过程中的介电行为，这两种突变体在限制温度下分别在细胞分离前和分离后停滞。出芽前但细胞分离后的单个球形细胞表现出一个主要的介电色散（中心频率约为1 MHz）和一个额外的介电色散（约20 MHz），它们分别是由于质膜以及细胞壁和液泡共同作用产生的麦克斯韦-瓦格纳色散。对于在细胞分离前立即积累成双细胞的细胞，除了与无芽单细胞相似的两个介电色散外，在200 kHz左右还出现了一个介电色散。这两种细胞介电行为的差异可能主要归因于细胞形状的差异，使用非球形细胞模型对此进行了理论研究。
