Watanabe M, Kono T, Koyama K, Sugimura T, Wakabayashi K
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1998 May;89(5):556-61. doi: 10.1111/j.1349-7006.1998.tb03297.x.
A substance strongly cytotoxic to human carcinoma cell line TMK-1 has been found in pupae, larvae and adults of the cabbage butterfly, Pieris rapae, and named pierisin. Pierisin was purified from the pupae of P. rapae by ammonium sulfate precipitation followed by DEAE-cellulose, Phenyl-Sepharose and hydroxyapatite column chromatographies. The molecular weight of the purified pierisin, which was homogenous on SDS-polyacrylamide gel, was analyzed by mass spectrometry and found to be 98 kDa. Pierisin showed a strong cytotoxic effect, with and IC50 of 0.75 ng/ml for human gastric carcinoma TMK-1 cells. The dying cells exhibited characteristic morphological features of apoptosis, such as cell shrinkage, chromatin condensation and nuclear fragmentation. Oligonucleosomal DNA fragmentation was also observed in DNA isolated from pierisin-treated cells. Moreover, similar characteristic changes showing apoptotic cell death were observed in TMK-1 cells treated with a crude extract of pupae of P. rapae. These results indicate that pierisin from the pupae of P. rapae induces apoptosis in human carcinoma cells.
在粉纹夜蛾的蛹、幼虫和成虫中发现了一种对人癌细胞系TMK-1具有强细胞毒性的物质，并将其命名为粉纹夜蛾素。通过硫酸铵沉淀，然后进行DEAE-纤维素、苯基琼脂糖和羟基磷灰石柱色谱法，从粉纹夜蛾的蛹中纯化出粉纹夜蛾素。通过质谱分析纯化后的粉纹夜蛾素（在SDS-聚丙烯酰胺凝胶上呈均一性）的分子量，发现其为98 kDa。粉纹夜蛾素表现出强烈的细胞毒性作用，对人胃癌TMK-1细胞的IC50为0.75 ng/ml。死亡细胞呈现出凋亡的特征性形态学特征，如细胞收缩、染色质浓缩和核碎裂。在用粉纹夜蛾素处理的细胞分离的DNA中也观察到了寡核小体DNA片段化。此外，在用粉纹夜蛾蛹的粗提物处理的TMK-1细胞中也观察到了显示凋亡细胞死亡的类似特征性变化。这些结果表明，粉纹夜蛾蛹中的粉纹夜蛾素可诱导人癌细胞凋亡。
