[使用间接免疫荧光和流式细胞术技术对小鼠抗IgG-荧光素偶联物的评估]

[Evaluation of a mouse anti-IgG-fluorescein conjugate using indirect immunofluorescence and flow cytometry techniques].

作者信息

Vilaseca J C, Pérez L, Savón C, Chacón D, Fernández S O, Rodríguez H, Goyenechea A, Otero A

机构信息

Instituto de Medicina Tropical Pedro Kourí.

出版信息

Rev Cubana Med Trop. 1997;49(2):120-4.

PMID:9685974

Abstract

An immunoglobulin G of mouse was purified from sera by affinity chromatography in protein A. The rabbits whose sera were able to recognize the antigen injected by double immunodiffusion were immunized with this preparation. The antibodies were precipitated from the rabbit's serum and purified by ion exchange chromatography. This preparation was conjugated to fluorescin isothiocyanate according to the conventional technique. The conjugated obtained was evaluated with the reference strains of Parainfluenza virus 1, 2, 3; Adenovirus; respiratory syncytial virus; and influenza virus A and B, by an indirect immunofluorescence technique and HIV positive samples by flow citometry. Specific monoclonal antibodies were used in both cases. Clinical specimens of patients with acute respiratory infection were evaluated.

摘要

从小鼠血清中通过蛋白A亲和层析纯化出一种免疫球蛋白G。用双免疫扩散法检测血清能识别所注射抗原的兔子，用该制剂进行免疫。抗体从兔血清中沉淀出来，并通过离子交换层析进行纯化。按照常规技术将该制剂与异硫氰酸荧光素结合。通过间接免疫荧光技术，用1型、2型、3型副流感病毒、腺病毒、呼吸道合胞病毒以及甲型和乙型流感病毒的参考毒株对所得结合物进行评估，并用流式细胞术对HIV阳性样本进行评估。两种情况均使用特异性单克隆抗体。对急性呼吸道感染患者的临床标本进行了评估。