Ben Rhouma K, Schimchowitsch S, Stoeckel M E, Felix J M, Sakly M
Laboratoire de Physiologie Animale, Faculté des Sciences de Bizerte, Tunisie, France.
Ann Endocrinol (Paris). 1997;58(6):475-81.
We have demonstrated in prior work that the density of cytoplasm receptors was comparable in DEXA-resistant and DEXA-sensible thymocyte populations (1). The present study was aimed at examining whether the resistance of thymocytes to the lytic action of glucocorticoids was linked or not to variation in the physico-chemical properties of the receptor itself. Our results show that the cytoplasmic receptors of DEXA-resistant thymocytes were, in terms of specificity, stability and elution profile on DEAE Sephacel columns, similar to those of DEXA-sensible cells. However, under condition of identical concentrations of DNA and receptors, we revealed a decrease in the nuclear transfer of glucocorticoid receptor in the DEXA-resistant thymocytes. This deficiency may account in part for the glucocorticoid resistance of these cells which may be related to intrathymic cell differentiation phenomenon.
我们在之前的研究中已经证明，地塞米松抵抗型和地塞米松敏感型胸腺细胞群体中细胞质受体的密度相当(1)。本研究旨在检验胸腺细胞对糖皮质激素溶解作用的抗性是否与受体本身物理化学性质的变化有关。我们的结果表明，就特异性、稳定性和在DEAE Sephacel柱上的洗脱图谱而言，地塞米松抵抗型胸腺细胞的细胞质受体与地塞米松敏感型细胞的相似。然而，在DNA和受体浓度相同的条件下，我们发现地塞米松抵抗型胸腺细胞中糖皮质激素受体的核转移减少。这种缺陷可能部分解释了这些细胞的糖皮质激素抗性，这可能与胸腺内细胞分化现象有关。
