Darrouzet V, Boudard P, Aran J M, Baquey C, Fizet D, Guilhaume A, Aurousseau C, Erre J P, Bebear J P
Service ORL, Hôpital Pellegrin, Bordeaux.
Ann Otolaryngol Chir Cervicofac. 1997;114(7-8):284-91.
Using the guinea pig middle ear model, we assessed decalcified, lyophylized, sterile heterotopic porcine ossicular xeno-implants based on a histology (optic and electron scan microscope) and immunologic (immunofluorscence) methods. Implants were placed in the middle ear and others in the dorsal subcutaneous area. Allo-implants were compared as controls. Implants were placed in the middle ear in 54 animals and skin implants in 14. Under the influence of BMP, the implant ossified in all cases in the middle ear. Intense immune recruitment was not observed. Inversely, there was a mononuclear infiltration reaction to the skin implants with formation of a fibrous capsule, immunoglobulin and complement influx and consequently sequestration. The allo-implants were partially reossified. These findings confirm the value of decalcification with hydrochloric acid for BMP induction, independent of species and the failure of attempted immune despecification. Implant outcome is not dependent on its antigen load, which is high compared with its weight, but on the site of implantation. The middle ear appears to be a privileged site of implantation.
利用豚鼠中耳模型，我们基于组织学（光学和电子扫描显微镜）及免疫学（免疫荧光）方法，对脱钙、冻干、无菌的异位猪听小骨异种移植物进行了评估。将移植物植入中耳，其他的植入背部皮下区域。以同种异体移植物作为对照。54只动物的移植物被植入中耳，14只动物的移植物被植入皮肤。在骨形态发生蛋白（BMP）的影响下，中耳内的移植物在所有情况下均发生骨化。未观察到强烈的免疫募集现象。相反，皮肤移植物出现单核细胞浸润反应，形成纤维囊，有免疫球蛋白和补体流入，进而发生隔离。同种异体移植物部分再骨化。这些发现证实了盐酸脱钙对BMP诱导的价值，该价值与物种无关，也证实了免疫去特异性尝试的失败。移植物的结果不取决于其抗原负荷（与其重量相比抗原负荷较高），而是取决于植入部位。中耳似乎是一个特殊的植入部位。
