[使用Ficoll-Paque（法玛西亚公司）和Gradisol L（波法公司）从骨髓、外周血和脐带血中分离人单核细胞。比较研究]
[Isolation of human mononuclear cells from bone marrow, peripheral blood and cord blood using Ficoll-Paque (Pharmacia) and Gradisol L (Polfa). Comparative study].
作者信息
Machaliński B, Honczarenko M, Gontarewicz A, Ratajczak M Z
机构信息
Zakładu Patologii Komórki Pomorskiej Akademii Medycznej.
出版信息
Pol Arch Med Wewn. 1998 Jan;99(1):15-23.
The aim of this study was to compare the effectiveness of Ficoll-Paque (Pharmacia), and Gradisol L (Polfa) solutions in isolating human mononuclear cells from: bone marrow, peripheral, and cord blood samples. We found employing both FACS analysis, and in vitro clonogenic tests for isolated cells, that both solutions allow to isolate identical cell populations. Therefore Gradisol-L (Polfa). which is much cheaper than Ficoll-Paque should be widely recommended in strategies for isolating hematopoietic mononuclear cells for both clinical and experimental purposes.
本研究的目的是比较菲可分离液（法玛西亚公司）和格拉迪索尔L（波尔法公司）溶液从骨髓、外周血和脐带血样本中分离人单核细胞的效果。我们通过对分离出的细胞进行流式细胞术分析和体外克隆形成试验发现，两种溶液都能分离出相同的细胞群体。因此，比菲可分离液便宜得多的格拉迪索尔L（波尔法公司），在用于临床和实验目的的造血单核细胞分离策略中应得到广泛推荐。
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