Downing L J, Strieter R M, Kadell A M, Wilke C A, Austin J C, Hare B D, Burdick M D, Greenfield L J, Wakefield T W
Department of Surgery, University of Michigan Medical Center, Ann Arbor 48109, USA.
J Immunol. 1998 Aug 1;161(3):1471-6.
Vein wall inflammation associated with venous thrombosis is mediated by an imbalance in proinflammatory as compared with antiinflammatory molecules. We hypothesize that IL-10 is an important antiinflammatory cytokine that influences vein wall inflammation and thrombus propagation during venous thrombosis. To test this hypothesis a model of inferior vena caval thrombosis was used. Studies were performed at sacrifice 2 days after thrombus induction and included leukocyte morphometrics, myeloperoxidase activity, vein wall permeability, thrombus weight, and IL-10 ELISA analysis from the vein wall. IL-10 was elevated in the vein wall during venous thrombosis. Neutralization of IL-10 increased inflammation, while supplementation with rIL-10 demonstrated a dose- and time-dependent decrease in inflammation. Interestingly, a low 2.5-microg rIL-10 dose given at time of initiation of thrombosis most significantly decreased inflammation. Thrombus weight was importantly diminished by reconstitution of IL-10. These studies support an important role for IL-10 in the regulation of thrombus-associated inflammation and thrombosis and suggest that IL-10 could be used as a therapeutic agent in the treatment of venous thrombosis.
与静脉血栓形成相关的静脉壁炎症是由促炎分子与抗炎分子失衡介导的。我们推测白细胞介素-10（IL-10）是一种重要的抗炎细胞因子，在静脉血栓形成过程中影响静脉壁炎症和血栓传播。为验证这一推测，采用了下腔静脉血栓形成模型。在血栓形成2天后处死动物进行研究，包括白细胞形态学、髓过氧化物酶活性、静脉壁通透性、血栓重量以及静脉壁的IL-10酶联免疫吸附测定分析。静脉血栓形成期间静脉壁中的IL-10升高。中和IL-10会增加炎症，而补充重组IL-10则显示出炎症呈剂量和时间依赖性降低。有趣的是，在血栓形成开始时给予低剂量2.5微克重组IL-10最显著地降低了炎症。补充IL-10可显著减轻血栓重量。这些研究支持IL-10在调节血栓相关炎症和血栓形成中起重要作用，并表明IL-10可作为治疗静脉血栓形成的治疗药物。
