自由生活变形虫福氏耐格里虫的基因变异。
Genetic variation in the free-living amoeba Naegleria fowleri.
作者信息
Pélandakis M, De Jonckheere J F, Pernin P
机构信息
Laboratoire de Biologie Cellulaire, Faculté de Pharmacie, 69373 Lyon cedex 08, France.
出版信息
Appl Environ Microbiol. 1998 Aug;64(8):2977-81. doi: 10.1128/AEM.64.8.2977-2981.1998.
In this study, 30 strains of the pathogenic free-living amoeba Naegleria fowleri were investigated by using the randomly amplified polymorphic DNA (RAPD) method. The present study confirmed our previous finding that RAPD variation is not correlated with geographical origin. In particular, Mexican strains belong to the variant previously detected in Asia, Europe, and the United States. In France, surprisingly, strains from Cattenom gave RAPD patterns identical to those of the Japanese strains. In addition, all of these strains, together with an additional French strain from Chooz, exhibited similarities to South Pacific strains. The results also confirmed the presence of numerous variants in Europe, whereas only two variants were detected in the United States. The two variants found in the United States were different from the South Pacific variants. These findings do not support the previous hypothesis concerning the origin and modes of dispersal of N. fowleri.
在本研究中，运用随机扩增多态性DNA（RAPD）方法对30株致病性自由生活阿米巴福氏耐格里阿米巴进行了调查。本研究证实了我们之前的发现，即RAPD变异与地理起源无关。特别是，墨西哥菌株属于先前在亚洲、欧洲和美国检测到的变体。令人惊讶的是，在法国，来自卡特农的菌株产生的RAPD模式与日本菌株相同。此外，所有这些菌株，连同来自舒兹的另一株法国菌株，都与南太平洋菌株表现出相似性。结果还证实欧洲存在众多变体，而在美国仅检测到两个变体。在美国发现的这两个变体与南太平洋变体不同。这些发现不支持先前关于福氏耐格里阿米巴起源和传播方式的假设。
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