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采用Colilert和Enterolert方法,通过膜过滤法和多管发酵法对瑞典南部用于饮用水和沐浴水水质监测的水中大肠菌群、大肠杆菌和肠球菌进行检测的比较

Comparison of membrane filtration and multiple-tube fermentation by the colilert and enterolert methods for detection of waterborne coliform bacteria, Escherichia coli, and enterococci used in drinking and bathing water quality monitoring in southern sweden.

作者信息

Eckner K F

机构信息

KM Lab AB, 251 07 Helsingborg, Sweden.

出版信息

Appl Environ Microbiol. 1998 Aug;64(8):3079-83. doi: 10.1128/AEM.64.8.3079-3083.1998.

Abstract

A total of 338 water samples, 261 drinking water samples and 77 bathing water samples, obtained for routine testing were analyzed in duplicate by Swedish standard methods using multiple-tube fermentation or membrane filtration and by the Colilert and/or Enterolert methods. Water samples came from a wide variety of sources in southern Sweden (Skåne). The Colilert method was found to be more sensitive than Swedish standard methods for detecting coliform bacteria and of equal sensitivity for detecting Escherichia coli when all drinking water samples were grouped together. Based on these results, Swedac, the Swedish laboratory accreditation body, approved for the first time in Sweden use of the Colilert method at this laboratory for the analysis of all water sources not falling under public water regulations (A-krav). The coliform detection study of bathing water yielded anomalous results due to confirmation difficulties. E. coli detection in bathing water was similar by both the Colilert and Swedish standard methods as was fecal streptococcus and enterococcus detection by both the Enterolert and Swedish standard methods.

摘要

总共338份用于常规检测的水样,其中261份饮用水样和77份沐浴水样,采用多管发酵法或膜过滤法,通过瑞典标准方法以及Colilert和/或Enterolert方法进行了双份分析。水样来自瑞典南部(斯科讷)的多种水源。当所有饮用水样归为一组时,发现Colilert方法在检测大肠菌群方面比瑞典标准方法更灵敏,而在检测大肠杆菌方面灵敏度相当。基于这些结果,瑞典实验室认可机构瑞典认可委员会首次在瑞典批准该实验室使用Colilert方法分析所有不属于公共用水法规(A类要求)范围内的水源。由于确认困难,沐浴水的大肠菌群检测研究得出了异常结果。Colilert方法和瑞典标准方法在检测沐浴水中大肠杆菌方面的结果相似,Enterolert方法和瑞典标准方法在检测粪链球菌和肠球菌方面的结果也相似。

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