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腐殖质组分和黏土对从土壤中分离出的荧光假单胞菌菌株降解菲的影响。

Effect of humic fractions and clay on biodegradation of phenanthrene by a Pseudomonas fluorescens strain isolated from soil.

作者信息

Ortega-Calvo J J, Saiz-Jimenez C

机构信息

Instituto de Recursos Naturales y Agrobiologia, C.S.I.C., Apartado 1052, 41080 Seville, Spain.

出版信息

Appl Environ Microbiol. 1998 Aug;64(8):3123-6. doi: 10.1128/AEM.64.8.3123-3126.1998.

Abstract

The mineralization of phenanthrene in pure cultures of a Pseudomonas fluorescens strain, isolated from soil, was measured in the presence of soil humic fractions and montmorillonite. Humic acid and clay, either separately or in combination, shortened the acclimation phase. A higher mineralization rate was measured in treatments with humic acid at 100 microg/ml. Humic acid at 10 microg/ml stimulated the transformation only in the presence of 10 g of clay per liter. We suggest that sorption of phenanthrene to these soil components may result in a higher concentration of substrate in the vicinity of the bacterial cells and therefore may increase its bioavailability.

摘要

在从土壤中分离出的荧光假单胞菌菌株的纯培养物中,在存在土壤腐殖质组分和蒙脱石的情况下测定了菲的矿化作用。腐殖酸和黏土单独或联合使用均缩短了驯化阶段。在腐殖酸浓度为100微克/毫升的处理中测得较高的矿化率。仅在每升含有10克黏土的情况下,10微克/毫升的腐殖酸才刺激转化作用。我们认为,菲吸附到这些土壤组分上可能导致细菌细胞附近底物浓度更高,因此可能会提高其生物可利用性。

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