Ortega-Calvo J J, Saiz-Jimenez C
Instituto de Recursos Naturales y Agrobiologia, C.S.I.C., Apartado 1052, 41080 Seville, Spain.
Appl Environ Microbiol. 1998 Aug;64(8):3123-6. doi: 10.1128/AEM.64.8.3123-3126.1998.
The mineralization of phenanthrene in pure cultures of a Pseudomonas fluorescens strain, isolated from soil, was measured in the presence of soil humic fractions and montmorillonite. Humic acid and clay, either separately or in combination, shortened the acclimation phase. A higher mineralization rate was measured in treatments with humic acid at 100 microg/ml. Humic acid at 10 microg/ml stimulated the transformation only in the presence of 10 g of clay per liter. We suggest that sorption of phenanthrene to these soil components may result in a higher concentration of substrate in the vicinity of the bacterial cells and therefore may increase its bioavailability.
在从土壤中分离出的荧光假单胞菌菌株的纯培养物中,在存在土壤腐殖质组分和蒙脱石的情况下测定了菲的矿化作用。腐殖酸和黏土单独或联合使用均缩短了驯化阶段。在腐殖酸浓度为100微克/毫升的处理中测得较高的矿化率。仅在每升含有10克黏土的情况下,10微克/毫升的腐殖酸才刺激转化作用。我们认为,菲吸附到这些土壤组分上可能导致细菌细胞附近底物浓度更高,因此可能会提高其生物可利用性。