Patel A J, Honoré E, Maingret F, Lesage F, Fink M, Duprat F, Lazdunski M
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, Valbonne, France.
EMBO J. 1998 Aug 3;17(15):4283-90. doi: 10.1093/emboj/17.15.4283.
Aplysia S-type K+ channels of sensory neurons play a dominant role in presynaptic facilitation and behavioural sensitization. They are closed by serotonin via cAMP-dependent phosphorylation, whereas they are opened by arachidonic acid, volatile general anaesthetics and mechanical stimulation. We have identified a cloned mammalian two P domain K+ channel sharing the properties of the S channel. In addition, the recombinant channel is opened by lipid bilayer amphipathic crenators, while it is closed by cup-formers. The cytoplasmic C-terminus contains a charged region critical for chemical and mechanical activation, as well as a phosphorylation site required for cAMP inhibition.
感觉神经元的海兔S型钾通道在突触前易化和行为敏感化中起主导作用。它们通过环磷酸腺苷（cAMP）依赖性磷酸化被5-羟色胺关闭，而被花生四烯酸、挥发性全身麻醉剂和机械刺激打开。我们已经鉴定出一种克隆的哺乳动物双P结构域钾通道，它具有S通道的特性。此外，重组通道被脂质双分子层两亲性促皱剂打开，而被杯状形成剂关闭。胞质C末端包含对化学和机械激活至关重要的带电区域，以及cAMP抑制所需的磷酸化位点。
