从假蜜环菌中分离出的酶对黄曲霉毒素B1的解毒作用

Detoxification of aflatoxin B1 by enzymes isolated from Armillariella tabescens.

作者信息

Liu D L, Yao D S, Liang R, Ma L, Cheng W Q, Gu L Q

机构信息

Department of Applied Chemistry, Sun Yat-Sen University, GuangZhou, PR China.

出版信息

Food Chem Toxicol. 1998 Jul;36(7):563-74. doi: 10.1016/s0278-6915(98)00017-9.

DOI:10.1016/s0278-6915(98)00017-9

PMID:9687963

Abstract

Detoxification of aflatoxin B1 (AFB1) by Armillariella tabescens multienzyme, which was isolated from mycelium pellets of A. tabescens, was confirmed by thin-layer chromatography (TLC) and rat assay. The results of toxicology and pathology studies showed that toxicity of AFB1 was minimized after treatment with A. tabescens multienzyme. The result of the Ames test indicated that the mutagenic activity of multienzyme-treated AFB1 was greatly reduced (or inactivated) compared with that of untreated controls. TLC determinations showed that AFB1 at an initial concentration of 16 microM was completely detoxified (100%) by the fungal multienzyme. The infrared spectrum suggests that the multienzyme is responsible for opening the difuran ring of AFB1.

摘要

从亮菌菌丝球中分离得到的亮菌多酶对黄曲霉毒素B1（AFB1）的解毒作用，通过薄层色谱法（TLC）和大鼠试验得到证实。毒理学和病理学研究结果表明，用亮菌多酶处理后，AFB1的毒性降至最低。艾姆斯试验结果表明，与未处理的对照组相比，多酶处理后的AFB1诱变活性大大降低（或失活）。TLC测定表明，初始浓度为16微摩尔的AFB1被真菌多酶完全解毒（100%）。红外光谱表明，多酶负责打开AFB1的二呋喃环。