黄曲霉毒素B1的水溶性葡糖醛酸和硫酸盐共轭物的表征。2. 大鼠肝细胞原代培养研究。

Characterization of water-soluble glucuronide and sulphate conjugates of aflatoxin B1. 2. Studies in primary cultures of rat hepatocytes.

作者信息

Wei C I, Hsieh D P

出版信息

Food Chem Toxicol. 1985 Sep;23(9):821-5. doi: 10.1016/0278-6915(85)90282-0.

Abstract

Aflatoxin B1 and some of its metabolites were released from water-soluble aflatoxin conjugates isolated from rat primary hepatocyte cultures and hydrolysed by enzymes (beta-glucuronidase and sulphatase), by acid or by a combination of both treatments. The presence of AFB1 in the hydrolysates was detected on TLC plates, or indicated indirectly by the Ames mutagenicity assay. The aflatoxin conjugates were not mutagenic to Salmonella typhimurium strain TA98 in the presence of rat-liver S-9 mix. However, following enzymatic hydrolysis, the chloroform extract of the hydrolysate was highly mutagenic to the bacteria, indicating the presence of mutagenic AFB1. The conjugates AFB1-glucuronide and AFB1-sulphate are therefore produced from AFB1 in primary cultures of rat hepatocytes.

摘要

从大鼠原代肝细胞培养物中分离得到的水溶性黄曲霉毒素结合物，经酶（β-葡萄糖醛酸酶和硫酸酯酶）、酸或两种处理方式结合水解后，释放出黄曲霉毒素B1及其一些代谢产物。水解产物中黄曲霉毒素B1的存在通过薄层色谱板进行检测，或通过艾姆斯致突变试验间接表明。在存在大鼠肝脏S-9混合液的情况下，黄曲霉毒素结合物对鼠伤寒沙门氏菌TA98菌株无致突变性。然而，酶解后，水解产物的氯仿提取物对该细菌具有高度致突变性，表明存在具有致突变性的黄曲霉毒素B1。因此，黄曲霉毒素B1-葡萄糖醛酸苷和黄曲霉毒素B1-硫酸酯结合物是在大鼠肝细胞原代培养物中由黄曲霉毒素B1产生的。