Tanaka T, Ikebuchi H, Sawada J, Tanaka Y
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Tokyo, Japan.
Lipids. 1998 Jul;33(7):733-6. doi: 10.1007/s11745-998-0264-0.
To obtain a specific antiserum for use in enzyme-linked immunosorbent assay (ELISA) of 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF), we prepared a hapten-carrier conjugate in which the CMPF hapten was linked to a carrier protein through the 5-(1-hydrazopropyl) group. The antisera raised against this antigen in guinea pigs had excellent specificity for CMPF, showing little cross-reactivity with closely related compounds and no significant cross-reactivities with other furan compounds. The results indicated that a specific antiserum to CMPF could be produced by an antigen whose CMPF moiety is linked to a carrier protein through a position remote from the inherent functional groups. A standard curve of CMPF by ELISA using a chemiluminescence system showed a high sensitivity and a linearity in the range of 5-100 ng/mL.
为了获得用于3-羧基-4-甲基-5-丙基-2-呋喃丙酸（CMPF）酶联免疫吸附测定（ELISA）的特异性抗血清，我们制备了一种半抗原-载体偶联物，其中CMPF半抗原通过5-(1-肼基丙基)基团与载体蛋白相连。在豚鼠中针对该抗原产生的抗血清对CMPF具有优异的特异性，与密切相关的化合物几乎没有交叉反应，与其他呋喃化合物也没有明显的交叉反应。结果表明，通过其CMPF部分通过远离固有官能团的位置与载体蛋白相连的抗原可以产生针对CMPF的特异性抗血清。使用化学发光系统的ELISA法测定CMPF的标准曲线在5-100 ng/mL范围内显示出高灵敏度和线性。
