Weber F, Huber S, Aloisi F, Meinl E
Department of Neuroimmunology, Max Planck Institute of Psychiatry, Martinsried, Germany.
J Neuroimmunol. 1998 Aug 1;88(1-2):99-104. doi: 10.1016/s0165-5728(98)00093-9.
In experimental allergic encephalomyelitis (EAE) myelin basic protein (MBP) specific T cells differ in their encephalitogenic potential. To investigate the functional diversity of human MBP specific T cell lines, we analysed their cytotoxic activity against human astrocytes and monocytes. Five out of 14 MBP specific T cell lines killed astrocytes in the presence of MBP. Nevertheless, all lines lysed blood derived monocytes. T cell lines that lysed astrocytes efficiently in the presence of MBP, recognized peptide aa 80-99/86-105 in context with HLA-DRB5 * 0101, peptide aa 50-69/61-83 in context with HLA-DRB1 * 1501 and peptides aa 139-153, and aa 148-162 in context with HLA-DRB1 * 0101. There was no correlation of MBP-mediated lysis of astrocytes with TCR-Vbeta usage, HLA-restriction and the production of tumor-necrosis-factor-alpha (TNF-alpha), lymphotoxin (LT) and interferon-gamma (IFN-gamma). Different lysis of astrocytes, however, revealed a functional heterogeneity of MBP specific T cells, which was not observed by using monocytes as targets.
在实验性变应性脑脊髓炎（EAE）中，髓鞘碱性蛋白（MBP）特异性T细胞的致脑炎性潜能有所不同。为了研究人MBP特异性T细胞系的功能多样性，我们分析了它们对人星形胶质细胞和单核细胞的细胞毒性活性。14个MBP特异性T细胞系中有5个在存在MBP的情况下可杀伤星形胶质细胞。然而，所有细胞系均可裂解来源于血液的单核细胞。在存在MBP的情况下能有效裂解星形胶质细胞的T细胞系，在与HLA-DRB5 * 0101结合时识别肽段aa 80 - 99/86 - 105，在与HLA-DRB1 * 1501结合时识别肽段aa 50 - 69/61 - 83，以及在与HLA-DRB1 * 0101结合时识别肽段aa 139 - 153和aa 148 - 162。MBP介导的星形胶质细胞裂解与TCR-Vβ使用、HLA限制以及肿瘤坏死因子-α（TNF-α）、淋巴毒素（LT）和干扰素-γ（IFN-γ）的产生之间没有相关性。然而，对星形胶质细胞的不同裂解揭示了MBP特异性T细胞的功能异质性，而以单核细胞为靶标时未观察到这种异质性。
