MTT：序列组装质量控制的软件工具。
MTT: a software tool for quality control in sequence assembly.
作者信息
Veklerov E, Eeckman F H, Martin C H
机构信息
Lawrence Berkeley National Laboratory, University of California, Berkeley, USA.
出版信息
Microb Comp Genomics. 1996;1(3):179-84. doi: 10.1089/mcg.1996.1.179.
A large-scale sequencing project requires a tool to control the quality of the input data because a sizable number of trace data may be of low quality. If these data are allowed to enter the sequence assembly pipeline, harm will be done. Hence, it is important to detect such data as soon as possible. MTT (Move-Track-Trim) is a software package analyzing the quality of the lanes. It subjects each lane to a series of tests, and if a lane does not pass all tests, it is flagged as a "bad" lane. The use has a chance to examine both the "good" and the "bad" lanes and reclassify a "bad" lane as "good," or vice versa. Alternatively, the user may decide to retrack the gel or get rid of some lanes altogether. As a by-product of the analysis, MTT performs other useful functions. It trims the lanes and compresses the lane files and moves them to the directories where assembly is carried out. It also generates some useful statistics describing the quality of the gel.
一个大规模测序项目需要一种工具来控制输入数据的质量，因为相当数量的痕量数据可能质量较低。如果允许这些数据进入序列组装流程，将会造成损害。因此，尽快检测出此类数据很重要。MTT（移动-追踪-修剪）是一个分析泳道质量的软件包。它对每个泳道进行一系列测试，如果某个泳道未通过所有测试，它就会被标记为“不良”泳道。用户有机会检查“良好”和“不良”泳道，并将“不良”泳道重新分类为“良好”，反之亦然。或者，用户可以决定重新追踪凝胶或完全舍弃某些泳道。作为分析的副产品，MTT还执行其他有用的功能。它修剪泳道、压缩泳道文件并将它们移动到进行组装的目录中。它还会生成一些描述凝胶质量的有用统计数据。
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