Mookerjee B K
Transplantation. 1976 Aug;22(2):101-7. doi: 10.1097/00007890-197608000-00004.
The effect, if any, of three different lymphocyte separation techniques on the composition and functional characteristics of the purified cell suspensions has been studied. Each separation technique was shown to yield a cell population with highly specific and reproducible characteristics. The Ficoll-Hypaque technique led to good lymphocyte yields but low yields of sheep erythrocyte-rosetting (E-rosetting) T cells, and the separated cell population responded least well to phytohemagglutinin. The glass sand filtration technique led to lowest overall yield of small lymphocytes and of EAC-rosetting cells. There was significantly lower total yield of E-rosetting T cell as well, but the separated lymphocyte suspension had excellent purity, had relatively high percentage of E-rosetting T cells, and they responded extremely well to phytohemagglutinin (PHA) and pokeweed mitogen (PWM). The Technicon separation involving magneticremoval of phagocytic cells by exposure to iron particles consistently led to large yields of small lymphocytes with good purity, the largest total harvests of E-rosetting T cells, as well as EAC-rosetting cells while the separated population had the highest percentage of E-rosetting cells and responded very well to PHA and PWM. These results show that lymphocyte losses during purification are not nonspecific and that the choice of the separation technique profoundly affects the characteristics of the purified lymphocyte population obtainable.
研究了三种不同的淋巴细胞分离技术对纯化细胞悬液的组成和功能特性的影响（若有影响的话）。结果表明，每种分离技术都能产生具有高度特异性和可重复性特征的细胞群体。Ficoll-泛影葡胺技术能获得较高的淋巴细胞产量，但绵羊红细胞花环形成（E花环形成）T细胞的产量较低，且分离出的细胞群体对植物血凝素的反应最差。玻璃砂过滤技术导致小淋巴细胞和EAC花环形成细胞的总体产量最低。E花环形成T细胞的总产量也显著较低，但分离出的淋巴细胞悬液纯度极高，E花环形成T细胞的百分比相对较高，且它们对植物血凝素（PHA）和商陆有丝分裂原（PWM）的反应非常好。通过接触铁颗粒利用磁性去除吞噬细胞的Technicon分离技术始终能获得大量纯度良好的小淋巴细胞、E花环形成T细胞以及EAC花环形成细胞的最大总收获量，同时分离出的群体中E花环形成细胞的百分比最高，且对PHA和PWM的反应非常好。这些结果表明，纯化过程中的淋巴细胞损失并非非特异性的，分离技术的选择对可获得的纯化淋巴细胞群体的特性有深远影响。
