Gomariz-Zilber E, Wioland M, Mehrishi J N
Service de Biophysique, Faculté de Médecine Saint-Antoine, Paris, France.
Cell Biophys. 1990 Jan-Apr;16(1-2):55-69. doi: 10.1007/BF02989692.
The changes induced in the distribution of the electrophoretic mobility (EPM) of human peripheral blood lymphocytes (HPBL), by various methods used to prepare the lymphocyte suspensions and eliminate platelets from them, were investigated on blood samples collected from healthy individuals and thrombopenic patients. Data showed that the distribution of the lymphocyte EPMs, i.e., the "lymphocyte electrophoregram," was dependent on the method chosen to enrich the suspension in the cell type of interest. The relative percentages of the low and high mobility cells, the two main subpopulations defined by lymphocyte electrophoresis, were different. The most striking artifactual differences in the lymphocyte electrophoregram were induced by the method of elimination of platelets; the distribution was unimodal and asymmetric when thrombin was used and bimodal when the blood sample, or the lymphocyte suspension, was placed on ice for 30 min (as is the practice in some laboratories). The "split" of the lymphocyte electrophoregram was found to be reversible within 90 min. Similar changes were observed on lymphocyte suspensions and blood samples of thrombopenic patients when the step for the elimination of platelets was not involved.
对从健康个体和血小板减少症患者采集的血液样本进行研究，以探究通过各种制备淋巴细胞悬液并从中去除血小板的方法，人类外周血淋巴细胞（HPBL）电泳迁移率（EPM）分布所发生的变化。数据表明，淋巴细胞EPM的分布，即“淋巴细胞电泳图”，取决于为富集目标细胞类型的悬液所选择的方法。淋巴细胞电泳所定义的两个主要亚群，即低迁移率细胞和高迁移率细胞的相对百分比有所不同。淋巴细胞电泳图中最显著的人为差异是由血小板去除方法引起的；当使用凝血酶时，分布是单峰且不对称的，而当血液样本或淋巴细胞悬液在冰上放置30分钟时（一些实验室的做法），分布是双峰的。发现淋巴细胞电泳图的“分裂”在90分钟内是可逆的。当不涉及血小板去除步骤时，在血小板减少症患者的淋巴细胞悬液和血液样本上也观察到了类似变化。
