Saetang T, Suttijitpaisal P, Ratanabanangkoon K
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
J Nat Toxins. 1998 Feb;7(1):37-44.
A simple procedure to prepare the toxic components from Naja kaouthia venom for use as immunogens has been studied. The aim was to produce serum rich in antitoxins. By heating the venom (1-6 mg/ml) at 100 degrees C for 10 min at pH 5.0, at least 10 proteins with MW greater than 25,000 daltons were precipitated and removed. The toxic components, i.e., postsynaptic toxins, Direct Lytic Factor (DLF), and phospholipase A2 were relatively stable to this treatment; however, their activities were progressively lost as the heating time was prolonged. The LD50S of the heated (100 degrees C, 10 min) and the unheated venom were 0.37 and 0.325 mg/kg, respectively. As compared to the unheated venom, immunization of rabbits with the heated venom resulted in a 3.38-fold increase in precipitable antibodies against N. kaouthia toxin 3 and a 1.85-fold increase in neutralizing capacity. This toxin preparation should be useful as an immunogen or as a starting material for chemical modification prior to immunization in the production of potent therapeutic antiserum.
一项用于制备眼镜王蛇毒液中有毒成分以用作免疫原的简单程序已得到研究。目的是生产富含抗毒素的血清。通过在pH 5.0条件下将毒液（1 - 6毫克/毫升）于100摄氏度加热10分钟，至少10种分子量大于25,000道尔顿的蛋白质沉淀并被去除。有毒成分，即突触后毒素、直接溶解因子（DLF）和磷脂酶A2对此处理相对稳定；然而，随着加热时间延长，它们的活性逐渐丧失。加热（100摄氏度，10分钟）毒液和未加热毒液的半数致死量分别为0.37和0.325毫克/千克。与未加热毒液相比，用加热毒液免疫兔子后，针对眼镜王蛇毒素3的可沉淀抗体增加了3.38倍，中和能力增加了1.85倍。这种毒素制剂应可作为免疫原或在生产高效治疗抗血清的免疫前用作化学修饰的起始材料。
