Liau M Y
Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1978 Dec;11(4):117-21.
As the preparation of high titer antiserum against Naja naja atra venom is a time-consuming process, attempts were made to develop a immunization procedure for producing highly potent antiserums within a short period. Rabbits were immunized for 12 weeks with (1) whole venom as used routinely in our Institute, (II) whole venom adsorbed on carboxymethyl-cellulose (CMC) and mixed with Freund's complete adjuvant and (III) neurotoxin adsorbed on CMC and adjuvant mixture followed by CMC-whole venom-adjuvant mixture. The results showed that one ml of the antiserum prepared by method (III) could neutralize 445 LD50 of whole venom, in other words, its potency was 4 and 40 times higher than those prepared by methods (II) and (I), respectively.
由于制备针对眼镜蛇毒的高效价抗血清是一个耗时的过程，因此人们尝试开发一种免疫程序，以便在短时间内生产出高效的抗血清。用以下方法对兔子进行了12周的免疫：（1）我们研究所常规使用的全毒液；（II）吸附在羧甲基纤维素（CMC）上并与弗氏完全佐剂混合的全毒液；（III）吸附在CMC和佐剂混合物上的神经毒素，随后是CMC-全毒液-佐剂混合物。结果表明，通过方法（III）制备的1毫升抗血清可以中和445个全毒液的半数致死量（LD50），换句话说，其效价比通过方法（II）和（I）制备的抗血清分别高4倍和40倍。
