Sarasquete C, González de Canales M L, Arellano J, Muñoz Cueto J A, Ribeiro L, Dinis M T
Institut of Sea Sciences of Andalucía, C.SI.C. Polígono Rio San Pedro, Puerto Real, Cádiz, Spain.
Histol Histopathol. 1998 Jul;13(3):727-35. doi: 10.14670/HH-13.727.
A battery of horseradish peroxidase-conjugated lectins (Con A, WGA and DBA), as well as conventional histochemical techniques (PAS, saponification, Alcian Blue pH 0.1, 1, 2.5, chlorhydric hydrolisis, neuraminidase, Bromophenol blue, Tioglycollate reduction and Ferric-ferricyanide-FeIII) were used to study the content and distribution of carbohydrates, proteins and glycoconjugate sugar residues on the skin and gills of Senegal sole, Solea senegalensis larvae and adults: During larval development of Solea senegalensis (from hatching until day 45 posthatching), epidermal sacciform, as well as branchial and epidermal chloride cells were unreactive with all cytochemical tests performed in this paper. Mucous or goblet cells of the corporal skin and gills containing strongly sulphated acid glycoproteins were evident on days 15-20 of larval development, as well as in epidermal and branchial mucous cells of adult specimens, which also contained GlcNAc and/or sialic acid. In adult specimen, the proteic content was higher in branchial mucous cells than in epidermal cells. In larvae, variable amounts of glycoproteins containing sialic acid, GlcNAc, GalNAc, Man and/or Glc residues were observed in epithelial cells and/or cuticle. GlcNAc and/or sialic acid sugar residues were only weakly detected in glycoproteins of some epidermal and branchial mucous cells of larvae by day 45, because from hatching until metamorphosis, lectin reactions (WGA, Con A and DBA) were negative in mucous cells.
使用一系列辣根过氧化物酶偶联凝集素（刀豆球蛋白A、小麦胚凝集素和双花扁豆凝集素）以及传统组织化学技术（过碘酸雪夫反应、皂化反应、pH 0.1、1、2.5的阿尔辛蓝染色、盐酸水解、神经氨酸酶处理、溴酚蓝染色、巯基乙醇还原和铁氰化铁-FeIII）来研究塞内加尔鳎（Solea senegalensis）幼体和成体皮肤及鳃上碳水化合物、蛋白质和糖缀合物糖残基的含量及分布：在塞内加尔鳎的幼体发育过程中（从孵化至孵化后45天），表皮囊状细胞以及鳃和表皮氯化物细胞对本文所进行的所有细胞化学测试均无反应。在幼体发育的第15 - 20天，以及成年标本的表皮和鳃黏液细胞中，含有强硫酸化酸性糖蛋白的体皮肤和鳃的黏液或杯状细胞明显可见，这些细胞还含有N - 乙酰葡糖胺和/或唾液酸。在成年标本中，鳃黏液细胞中的蛋白质含量高于表皮细胞。在幼体中，在上皮细胞和/或角质层中观察到含有唾液酸、N - 乙酰葡糖胺、N - 乙酰半乳糖胺、甘露糖和/或葡萄糖残基的不同量糖蛋白。到45天时，在幼体的一些表皮和鳃黏液细胞的糖蛋白中仅微弱检测到N - 乙酰葡糖胺和/或唾液酸糖残基，因为从孵化到变态，黏液细胞中的凝集素反应（小麦胚凝集素、刀豆球蛋白A和双花扁豆凝集素）均为阴性。
