Russell N S, Grummels A, Hart A A, Smolders I J, Borger J, Bartelink H, Begg A C
The Netherlands Cancer Institute/Antoni van Leeuwenhoekhuis, Amsterdam.
Int J Radiat Biol. 1998 Jun;73(6):661-70. doi: 10.1080/095530098141915.
To test whether the intrinsic radiosensitivity of skin fibroblasts from breast cancer patients correlates with the degree of breast fibrosis after breast conserving therapy.
In a systematic study design, 79 patients were selected from an earlier study group of 385 patients based on observed fibrosis and seven identified clinical risk factors for fibrosis development. In vitro radiosensitivity of patients' dermal fibroblasts was determined by clonogenic assay of early passage cultures. Survival was determined after irradiation at 0, 2 and 4 Gy, given in two fractions of 2 Gy with a 6 h interval.
There was a significant inter-patient variation for SF2 values (coefficient of variation = 40%). The ratio of SF2 values for fibroblasts from patients with breast fibrosis versus those without was 0.80 (95% CI: 0.60-1.07). This was a statistically non-significant trend (p = 0.13). The same ratio for a derived value for SF2 ((SF2 + square root of SF4)/2) was 0.88 (p = 0.19).
A significant variation in intrinsic radiosensitivity of breast cancer patients' dermal fibroblasts was observed. However, the degree of radiosensitivity did not show a significant correlation with fibrosis development. This indicates that the use of fibroblast radiosensitivity will have a limited usefulness for predicting fibrosis following breast irradiation.
检测乳腺癌患者皮肤成纤维细胞的内在放射敏感性是否与保乳治疗后乳腺纤维化程度相关。
在一项系统研究设计中，基于观察到的纤维化情况以及七个已确定的纤维化发生临床风险因素，从早期的385例患者研究组中选取79例患者。通过早期传代培养物的克隆形成试验测定患者真皮成纤维细胞的体外放射敏感性。以2Gy的剂量分两次照射，间隔6小时，分别给予0、2和4Gy照射后测定细胞存活率。
患者间SF2值存在显著差异（变异系数=40%）。有乳腺纤维化患者的成纤维细胞与无乳腺纤维化患者的成纤维细胞的SF2值之比为0.80（95%可信区间：0.60 - 1.07）。这是一个统计学上无显著意义的趋势（p = 0.13）。对于SF2的一个衍生值（(SF2 + SF4的平方根)/2），相同的比值为0.88（p = 0.19）。
观察到乳腺癌患者真皮成纤维细胞的内在放射敏感性存在显著差异。然而，放射敏感性程度与纤维化发展未显示出显著相关性。这表明使用成纤维细胞放射敏感性来预测乳腺放疗后的纤维化作用有限。
