Kristofiková Z, Klaschka J, Tejkalová H
Prague Psychiatric Centre, Czech Republic.
Neurochem Res. 1998 Jul;23(7):923-9. doi: 10.1023/a:1021025302664.
The Na+-dependent high-affinity choline uptake (HACU) transport and the [3H]hemicholinium-3 ([3H]HC-3) specific binding were measured on hippocampal synaptosomes of young (3-6 months) and old (22 months) Wistar rats. In vitro effects of 100-300 microM arachidonic acid (AA) and of 5% ethanol were tested under basal as well as stimulated (55 mM KCl) conditions. The influence of AA (an irreversible decrease of HACU and a reversible increase of [3H]HC-3 binding) was more marked under stimulated rather than basal conditions in brain tissue of young rats. The increased K+-depolarization effect on HACU and the decreased influence of AA on [3H]HC-3 binding were estimated in brain tissue of old compared to young rats. Results suggest the involvement of different pools of the high-affinity choline carrier and marked changes due to aging in the regulation of the HACU transport.
在年轻（3至6个月）和老年（22个月）Wistar大鼠的海马突触体上测量了钠依赖性高亲和力胆碱摄取（HACU）转运和[3H]半胱氨酸-3（[3H]HC-3）特异性结合。在基础以及刺激（55 mM氯化钾）条件下测试了100至300 microM花生四烯酸（AA）和5%乙醇的体外作用。在年轻大鼠的脑组织中，刺激条件下AA对HACU的不可逆降低和对[3H]HC-3结合的可逆增加的影响比基础条件下更明显。与年轻大鼠相比，在老年大鼠的脑组织中估计了钾离子去极化对HACU的增强作用以及AA对[3H]HC-3结合的降低影响。结果表明高亲和力胆碱载体的不同池参与其中，并且衰老导致HACU转运调节发生显著变化。
