Fletcher O J, Anderson D P, Kleven S H
Vet Pathol. 1976;13(4):303-14. doi: 10.1177/030098587601300407.
Chickens were housed when 1 day old with chickens experimentally infected with Mycoplasma synoviae. Air sacs from 16 of the 2-week-old and eight of the 3-week-old contact-exposed chickens were given gross lesion scores and embedded in glycol methacrylate for slide preparation. Histologic lesions in air sacs with gross scores of 0-2 were mild edema resulting in a two to eightfold increase in air sac thickness, capillary proliferation, and exudate consisting largely of heterophils and necrotic debris. Histologic lesions in air sacs with gross scores of 3 and 4 were marked hyperplasia of epithelial cells and diffuse infiltration of the air sac connective tissue by mononuclear cells. Nine of 10 air sacs with gross scores of 0 had no histologic evidence of inflammation. The most severe histologic lesions were in those air sacs with gross scores of 4. The glycol methacrylate procedure resulted in 2-mum sections with excellent cellular detail.
1日龄雏鸡与经实验感染滑膜支原体的鸡饲养在一起。对16只2周龄和8只3周龄接触暴露鸡的气囊进行大体病变评分，并将其包埋在甲基丙烯酸乙二醇酯中以制备玻片。大体评分为0 - 2分的气囊组织学病变为轻度水肿，导致气囊厚度增加2至8倍、毛细血管增生以及主要由嗜异性粒细胞和坏死碎片组成的渗出物。大体评分为3分和4分的气囊组织学病变为上皮细胞明显增生以及单核细胞对气囊结缔组织的弥漫性浸润。10个大体评分为0分的气囊中有9个没有炎症的组织学证据。组织学病变最严重的是那些大体评分为4分的气囊。甲基丙烯酸乙二醇酯制片法得到了细胞细节清晰的2微米切片。
