Danty E, Arnold G, Burmester T, Huet J C, Huet D, Pernollet J C, Masson C
Neurobiologie Expérimentale et Théorie des Systèmes complexes, CNRS UPR 9081, Paris, France.
Insect Biochem Mol Biol. 1998 May-Jun;28(5-6):387-97. doi: 10.1016/s0965-1748(98)00011-3.
Four distinct hexamerin subunits (referred to as "hexamerins" in the following text) have been identified in the developing honeybee, Apis mellifera, by N-terminal protein sequencing. Hexamerins are abundant in the hemolymph of late larval and early pupal stages, and gradually decline during metamorphosis and adult development. Three hexamerins in the 70 kDa range have been found (Hex70a, Hex70b, Hex70c). In worker and drone, Hex70a is the only hexamerin present in large amount in later adult stages. Hex70b and c exhibit a similar developmental profile, disappearing in the drone just before adult emergence, and in the worker just after. Hex70b or Hex70c are still detectable in the adult queen. Hex80/110 likely exist in at least 3 different subunits, 1 of 110 kDa, and 2 of around 80 kDa, which all share a common N-terminus. They disappear during metamorphosis earlier than Hex70b and c. All these hexamerins have been found also in the antenna, suggesting their utilization in building up of antennal cuticle structures.
通过N端蛋白质测序，在发育中的蜜蜂（西方蜜蜂）中鉴定出了四种不同的六聚蛋白亚基（在下文简称为“六聚蛋白”）。六聚蛋白在幼虫后期和蛹期早期的血淋巴中含量丰富，在变态发育和成虫发育过程中逐渐减少。已发现三种分子量在70 kDa左右的六聚蛋白（Hex70a、Hex70b、Hex70c）。在工蜂和雄蜂中，Hex70a是成虫后期大量存在的唯一六聚蛋白。Hex70b和Hex70c呈现出相似的发育模式，在雄蜂成虫羽化前消失，在工蜂成虫羽化后消失。Hex70b或Hex70c在成年蜂王中仍可检测到。Hex80/110可能至少存在3种不同的亚基，一种为110 kDa，另外两种约为80 kDa，它们都有一个共同的N端。它们在变态发育过程中比Hex70b和Hex70c更早消失。所有这些六聚蛋白在触角中也被发现，这表明它们在触角表皮结构的构建中发挥作用。
