Domingues M R, Nemirovskiy O V, Marques M G, Neves M G, Cavaleiro J A, Ferrer-Correia A J, Gross M L
Department of Chemistry, University of Aveiro, Portugal.
J Am Soc Mass Spectrom. 1998 Aug;9(8):767-74. doi: 10.1016/s1044-0305(98)00048-8.
High-energy (HE) and low-energy (LE) collisionally activated decompositions of octaethylporphyrin (OEP) and its metal complexes (ZnOEP and CuOEP) depend on whether the precursor is produced by electrospray ionization as protonated molecules or by fast atom bombardment as radical cations or protonated molecules. LE activation leads to such simple product-ion spectra that a complete picture of fragmentation emerges only after nine stages of tandem mass spectrometry (MS). HE activation, on the other hand, gives product-ion spectra that afford an integrated view of all the decomposition channels in a single MS/MS experiment. These results are the basis of a recommendation that OEP is an appropriate model compound for investigating energy effects in the collisional activation of organic and bioorganic molecule ions.
八乙基卟啉（OEP）及其金属配合物（ZnOEP和CuOEP）的高能（HE）和低能（LE）碰撞激活分解取决于前体是通过电喷雾电离作为质子化分子产生，还是通过快原子轰击作为自由基阳离子或质子化分子产生。低能激活产生的产物离子光谱非常简单，只有在串联质谱（MS）进行九个阶段后才会呈现出完整的碎片化图景。另一方面，高能激活产生的产物离子光谱能在单次MS/MS实验中提供所有分解通道的综合视图。这些结果为以下建议提供了依据：OEP是研究有机和生物有机分子离子碰撞激活中能量效应的合适模型化合物。
