大肠杆菌志贺毒素VT-1的B亚基同五聚体与球三糖基神经酰胺三糖部分之间复合物的溶液结构。

Solution structure of the complex between the B-subunit homopentamer of verotoxin VT-1 from Escherichia coli and the trisaccharide moiety of globotriaosylceramide.

作者信息

Shimizu H, Field R A, Homans S W, Donohue-Rolfe A

机构信息

Centre for Biomolecular Sciences, University of St. Andrews, FIFE, UK.

出版信息

Biochemistry. 1998 Aug 4;37(31):11078-82. doi: 10.1021/bi980946+.

DOI:10.1021/bi980946+

PMID:9693003

Abstract

We report the solution structure of the carbohydrate-binding B subunit of verotoxin VT-1 (VTB) from enterohemorrhagic Escherichia coli in association with the trisaccharide Galalpha1-4Galbeta1-4Glcbeta1-O-trimethylsilylethyl , determined by use of stable isotope-assisted NMR techniques. In contrast to the crystal structure of the complex which predicts three binding sites per monomer, only one of these sites is observed with substantial occupancy by the trisaccharide in solution.

摘要

我们报道了肠出血性大肠杆菌中维罗毒素VT-1（VTB）的碳水化合物结合B亚基与三糖Galα1-4Galβ1-4Glcβ1-O-三甲基硅烷基乙基结合的溶液结构，该结构通过使用稳定同位素辅助的核磁共振技术确定。与预测每个单体有三个结合位点的复合物晶体结构不同，在溶液中三糖仅大量占据其中一个位点。