Kobayashi T, Koshida K, Endo Y, Imao T, Uchibayashi T, Sasaki T, Namiki M
Department of Urology, School of Medicine, Kanazawa University, Kanazawa, Japan.
Eur Urol. 1998 Aug;34(2):154-60. doi: 10.1159/000019702.
To establish a suitable experimental model of bone and liver micrometastases from human prostate cancer for evaluating antitumor agents.
PC-3 cells, an androgen-independent prostate cancer cell line, were inoculated into the chorioallantoic membrane vein of 10-day-old chick embryos (10(6) cells/egg). The polymerase chain reaction product for the human beta-globin gene in chick embryo femur and liver was quantified at various time points after inoculation, when immunohistochemical staining was done for Ki-67 antigen and cytokeratin. The antitumor effect of suramin was evaluated using the model, as was regional blood flow after thallium injection.
Micrometastases were identified in bone and liver 1 day after inoculation and grew to form established metastatic foci in all embryos. Suramin showed significant antitumor effect for liver metastases, but not for those in bone where blood flow was relatively low.
The chick embryo system provides a highly reproducible model for bone and liver micrometastases from human prostate cancer, suitable for evaluating antineoplastic agents at an early stage of the metastatic process.
建立一种合适的人前列腺癌骨和肝微转移实验模型，用于评估抗肿瘤药物。
将雄激素非依赖性前列腺癌细胞系PC-3细胞接种到10日龄鸡胚的绒毛尿囊膜静脉中（每枚鸡蛋接种10⁶个细胞）。接种后不同时间点对鸡胚股骨和肝脏中的人β-珠蛋白基因的聚合酶链反应产物进行定量，同时对Ki-67抗原和细胞角蛋白进行免疫组织化学染色。使用该模型评估苏拉明的抗肿瘤作用以及注射铊后的局部血流情况。
接种后1天在骨和肝中发现微转移，且在所有胚胎中均生长形成了已确立的转移灶。苏拉明对肝转移显示出显著的抗肿瘤作用，但对血流相对较低的骨转移则无此作用。
鸡胚系统为人类前列腺癌的骨和肝微转移提供了一种高度可重复的模型，适用于在转移过程的早期阶段评估抗肿瘤药物。
