Kimitsuki T, Taira T, Komune S, Komiyama S
Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
ORL J Otorhinolaryngol Relat Spec. 1998 Sep-Oct;60(5):263-6. doi: 10.1159/000027607.
Intracellular calcium concentration ([Ca2+]i) was elevated by photolysis of the caged calcium compound, Nitr-5, and by use of the intrapipette perfusion technique in hair cells dissociated from the chick cochlea. An increase in [Ca2+]i induced an outward-going current at a membrane potential of -40 mV, as recorded with an intracellular medium of 160 mM KCl. This current was carried by the Ca2+-activated K+ current. In contrast, an increase of [Ca2+]i induced an inward-going current at -50 mV with a 160 mM CsCl-based intracellular medium. This inward-going current was carried by the Ca2+-activated non-selective cation current. The amplitude of the mechano-electrical transduction current was suppressed by the increase of [Ca2+]i, achieved both by photolysis and by use of the intrapipette perfusion method.
通过光解笼锁钙化合物Nitr-5以及利用从鸡耳蜗分离出的毛细胞中的吸管内灌注技术，细胞内钙浓度（[Ca2+]i）升高。如用160 mM KCl的细胞内液记录，[Ca2+]i的增加在-40 mV的膜电位下诱导出外向电流。该电流由钙激活钾电流携带。相反，在基于160 mM CsCl的细胞内液中，[Ca2+]i的增加在-50 mV时诱导出内向电流。该内向电流由钙激活非选择性阳离子电流携带。通过光解和吸管内灌注方法实现的[Ca2+]i增加均抑制了机械电转导电流的幅度。
