耳毒性药物对雏鸡耳蜗毛细胞机械电转导通道的影响。

The effects of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells.

作者信息

Kimitsuki T, Nakagawa T, Hisashi K, Komune S, Uemura T

机构信息

Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.

出版信息

Eur Arch Otorhinolaryngol. 1994;251 Suppl 1:S53-6. doi: 10.1007/BF02565220.

DOI:10.1007/BF02565220

PMID:11894776

Abstract

The effects of ototoxic drugs on mechano-electrical transduction (MET) currents were investigated in dissociated cochlear hair cells of the chick, using a whole-cell patch-electrode voltage-clamp technique. Dihydrostreptomycin (DHSM) and cisplatin (cis-dichlorodiammine platinum II, CDDP) blocked the MET channel in a dose- and voltage-dependent manner. In contrast, acetyl salicylate did not suppress the MET current. At -50 mV, DHSM and CDDP blocked the MET channel with a Hill coefficient of 0.93 and 2.1, respectively. These findings suggest that a single DHSM molecule or more than one CDDP molecule binds to a single MET channel.

摘要

采用全细胞膜片钳电压钳技术，在雏鸡分离的耳蜗毛细胞中研究了耳毒性药物对机械电转导（MET）电流的影响。双氢链霉素（DHSM）和顺铂（顺式二氯二氨铂II，CDDP）以剂量和电压依赖性方式阻断MET通道。相比之下，乙酰水杨酸并未抑制MET电流。在-50 mV时，DHSM和CDDP分别以0.93和2.1的希尔系数阻断MET通道。这些发现表明，单个DHSM分子或多个CDDP分子与单个MET通道结合。