Yiangou M, Tsapogas P, Nikolaidis N, Scouras Z G
Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki (AUTH), Greece.
Cytobios. 1997;92(369):91-8.
Newly synthesized polypeptides during recovery from prolonged cold treatment (0 degree to -1 degree C) of Drosophila auraria, a montium subgroup species, of the melanogaster species group, were analysed in denaturing polyacrylamide gels. In addition, during the cold shock recovery period, Northern analysis of the hsp83 mRNA was performed. A significant induction of two polypeptides, which exhibited electrophoretic mobilities, with the heat inducible 83 and 70 kD hsp83 and hsp70 was detected, but no such induction was evident in the so-called 'small' hsp genes. These results are compared and discussed with those observed in D. melanogaster.
对黑腹果蝇种组山地亚组物种奥瑞利亚果蝇在从长时间冷处理（0摄氏度至零下1摄氏度）恢复过程中新合成的多肽进行了变性聚丙烯酰胺凝胶分析。此外，在冷休克恢复期间，对hsp83 mRNA进行了Northern分析。检测到两种多肽有显著诱导现象，它们表现出电泳迁移率，与热诱导的83 kD hsp83和70 kD hsp70相似，但在所谓的“小”hsp基因中没有这种诱导现象。将这些结果与在黑腹果蝇中观察到的结果进行了比较和讨论。
